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Sigma-Aldrich Product Name EL4 Cell Line murine, 85023105, Lymphoblast from blood biological source
List Price $770.00 Your Price $770.00
Sigma-Aldrich Product Name EL4 Cell Line murine, 85023105, Lymphoblast from blood biological source mouse blood growth mode Suspension karyotype 2n = 39 morphology Lymphoblast products A surface - SIALUPENN (Additional S&H or Hazmat Fees may Apply)
NETA PART: SIALUPENN-85023105-1VL
MFG.PART: 85023105-1VL
UNSPSC: 41122409
Manufacturer: Sigma-Aldrich
Product Name
EL4 Cell Line murine, 85023105, Lymphoblast from blood
biological source
mouse blood
growth mode
Suspension
karyotype
2n = 39
morphology
Lymphoblast
products
A surface antigen induced by leukaemia type G virus; H-2b and Thy-1.2 antigens; Interleukin 4 (IL-4), H-2b, Thy1.2
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
Related Categories
Cell Culture & Analysis
Cell Lines & Specialty Cell Culture
Mammalian Cell Lines
Molecular Biology & Functional Genomics
Cell Line Origin
Mouse ascites lymphoma lymphoblast
Cell Line Description
Established from a lymphoma induced in a C57BL/6N mouse by 9,10-dimethyl-1,2-benzanthracene. The cells have been reported to produce low levels of type G (Gross) virus antigen histone-2b (H-2b), interleukin-4 (IL-4) and Thy-1.2 antigen. The cell line is resistant to cortisol and dexamethasone and is sensitive to polyhydroxyalkanoates (PHAs).
Application
EL4 Cell Line murine has been used to induce tumor in mice and to assess the extravasation of tumor necrosis factor (TNF)-induced Evans blue dye and E1- and E3-deleted Ad vectors carrying firefly luciferase reporter gene under cytomegalovirus promoter (AdLuc). It has also been used as a constituent of bone marrow-derived macrophage (BMDM)-polarizing media to polarize BMDM and assess its tumoricidal activity.[1][2]
Tumourigenicity studies
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Cell growth after resuscitation is slow. Subculture once exponential growth is established.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
| SKU | SIALUPENN-85023105-1VL |
|---|---|
| Supplier Part Number | 85023105-1VL |
| UM | EA |
| UNSPSC | 41122409 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIALUPENN |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |