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Sigma-Aldrich Product Name MRC-5 SV1 TG2, 85042502 biological source human lung (fetal) growth mode
List Price $436.00 Your Price $436.00
Sigma-Aldrich Product Name MRC-5 SV1 TG2, 85042502 biological source human lung (fetal) growth mode Adherent karyotype Modal no. = 60-80 hperdiploid morphology Fibroblast products Not specified - SIALUPENN (Additional S&H or Hazmat Fees may Apply)
NETA PART: SIALUPENN-85042502-DNA-5UG
MFG.PART: 85042502-DNA-5UG
UNSPSC: 41122409
Manufacturer: Sigma-Aldrich
Product Name
MRC-5 SV1 TG2, 85042502
biological source
human lung (fetal)
growth mode
Adherent
karyotype
Modal no. = 60-80 hperdiploid
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human foetal lung, SV40 transformed
Cell Line Description
A clonal derivative of an SV40 transformed permanent line of MRC-5. Selection was made using X-ray induction in the presence of 2μg/ml thioguanine. The cells are HAT sensitive.
DNA Profile
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,14
D16S539: 11
D5S818: 11,12
D7S820: 10
THO1: 8
TPOX: 8
vWA: 15
CSF1PO: 11,12
D13S317: 11,14
D16S539: 11
D5S818: 11,12
D7S820: 10
THO1: 8
TPOX: 8
vWA: 15
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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| SKU | SIALUPENN-85042502-DNA-5UG |
|---|---|
| Supplier Part Number | 85042502-DNA-5UG |
| UM | EA |
| UNSPSC | 41122409 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIALUPENN |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |