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Sigma-Aldrich Product Name F9, 85061803 biological source mouse embryo growth mode Adherent karyotyp
List Price
$436.00
Your Price
$436.00
Sigma-Aldrich Product Name F9, 85061803 biological source mouse embryo growth mode Adherent karyotype Not specified morphology Not specified products Not specified receptors Not specified - SIALUPENN (Additional S&H or Hazmat Fees may Apply)
NETA PART:
SIALUPENN-85061803-DNA-5UG
MFG.PART:
85061803-DNA-5UG
UNSPSC:
41122409
Manufacturer:
Sigma-Aldrich
Product Name
F9, 85061803
biological source
mouse embryo
growth mode
Adherent
karyotype
Not specified
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
Cell Line Origin
Mouse 129 embryo teratocarcinoma
Cell Line Description
Derived from testicular carcinoma. F9 undergo very limited differentiation under normal conditions but can be induced to differentiate into parietal endoderm in the presence of retinoic acid and dibutyryl cAMP.
Application
Differentiation studies
Culture Medium
DMEM + 2mM Glutamine + 10-15% Foetal Bovine Serum (FBS). Pre-soak flasks in 0.1% gelatin for over 2 hours and wash with PBS.
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2, 37°C. Upon resuscitation, cells shed large quantities of debris, leave for 2 days before subculture. Please note: Culture v
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
| SKU | SIALUPENN-85061803-DNA-5UG |
|---|---|
| Supplier Part Number | 85061803-DNA-5UG |
| UM | EA |
| UNSPSC | 41122409 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIALUPENN |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |