Sigma-Aldrich Product Name MA104, from African green monkey kidney, 85102918, Convention on the

Monkey African Green kidney

Established from an African green monkey foetal kidney. The cells are highly susceptible to Simian rotavirus SA11.

Please note: The species of origin of the MA104 cell line has been shown to be different from that claimed by the originators.

Whitaker & Hayward (1985) found the cell line to originate from an African green monkey and not a Rhesus macaque as was originally claimed. Whitaker AM and Hayward CJ (1985). The characterization of three monkey kidney cell lines. Develop. Biol. Standard. Vol 60: 125-131. PMID: 4043530. Abstract: Three monkey kidney cell lines, Vero, GL-V3 and MA-104 were subjected to karyological analysis to determine their chromosomal stability and to confirm their species of origin. Although the lines were shown to be relatively stable throughout all of the passage levels that were tested, the species of origin of one of them was found to be different from that claimed by the originators. This finding was supported by data from isoenzyme studies.

EMEM (EBSS) (M2279) + 2 mM L-Glutamine (G7513) + 1% NEAA (M7145) + 10% FBS / FCS (F2442).

Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.

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