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Sigma-Aldrich Product Name 208F, 85103116 biological source rat embryo growth mode Adherent karyotyp
List Price
$436.00
Your Price
$436.00
Sigma-Aldrich Product Name 208F, 85103116 biological source rat embryo growth mode Adherent karyotype Not specified morphology Fibroblast products Not specified receptors Not specified technique(s) - SIALUPENN (Additional S&H or Hazmat Fees may Apply)
NETA PART:
SIALUPENN-85103116-DNA-5UG
MFG.PART:
85103116-DNA-5UG
UNSPSC:
41122409
Manufacturer:
Sigma-Aldrich
Product Name
208F, 85103116
biological source
rat embryo
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Culture & Analysis
Cell Lines & Specialty Cell Culture
Mammalian Cell Lines
Molecular Biology & Functional Genomics
Cell Line Origin
Rat Fischer fibroblast
Cell Line Description
Derived from a subclone of a BUdR-resistant strain of a Fischer rat fibroblast 3T3-like line RAT-1. The line is suitable for transformation and TGF (SGF) assays as an indicator cell line.
Application
Transformation studies
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1% Vitamins + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
| SKU | SIALUPENN-85103116-DNA-5UG |
|---|---|
| Supplier Part Number | 85103116-DNA-5UG |
| UM | EA |
| UNSPSC | 41122409 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIALUPENN |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |