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Sigma-Aldrich Product Name STO, 86032003 biological source mouse embryo growth mode Adherent karyoty
List Price $436.00 Your Price $436.00
Sigma-Aldrich Product Name STO, 86032003 biological source mouse embryo growth mode Adherent karyotype Not specified morphology Fibroblast products Not specified receptors Not specified technique(s) - SIALUPENN (Additional S&H or Hazmat Fees may Apply)
NETA PART: SIALUPENN-86032003-DNA-5UG
MFG.PART: 86032003-DNA-5UG
UNSPSC: 41122409
Manufacturer: Sigma-Aldrich
Product Name
STO, 86032003
biological source
mouse embryo
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse SIM embryo fibroblast
Cell Line Description
Selected for 6-thioguanine and ouabain resistance; sensitive to HAT; HPRT negative. Used routinely as feeder layers prepared by irradiation or mitomycinc treatment for maintenance of teratocarcinoma stem cells in the undifferentiated state.
Application
Oubain resistance, feeder layer
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
| SKU | SIALUPENN-86032003-DNA-5UG |
|---|---|
| Supplier Part Number | 86032003-DNA-5UG |
| UM | EA |
| UNSPSC | 41122409 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIALUPENN |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |