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Sigma-Aldrich Product Name NRK-49F, 86101301 biological source rat kidney growth mode Adherent
List Price $436.00 Your Price $436.00
Sigma-Aldrich Product Name NRK-49F, 86101301 biological source rat kidney growth mode Adherent karyotype Not specified morphology Fibroblast products Not specified receptors Not specified - SIALUPENN (Additional S&H or Hazmat Fees may Apply)
NETA PART: SIALUPENN-86101301-DNA-5UG
MFG.PART: 86101301-DNA-5UG
UNSPSC: 41122409
Manufacturer: Sigma-Aldrich
Product Name
NRK-49F, 86101301
biological source
rat kidney
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Culture & Analysis
Cell Lines & Specialty Cell Culture
Mammalian Cell Lines
Molecular Biology & Functional Genomics
Cell Line Origin
Rat kidney fibroblast
Cell Line Description
Derived from normal kidney and exhibits contact-inhibition. It is sensitive to viral or chemical transformation, including proteins such as SGF. Originated from the same mixed culture as NRK-52E but has distinct characteristics.
Application
Contact inhibition and transformation studies
Culture Medium
DMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 5-10% Foetal Bovine Serum (FBS) or New Born Calf Serum (NBCS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. The cells should be maintained sub-confluent, otherwise they will transform.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
| SKU | SIALUPENN-86101301-DNA-5UG |
|---|---|
| Supplier Part Number | 86101301-DNA-5UG |
| UM | EA |
| UNSPSC | 41122409 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIALUPENN |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |