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Sigma-Aldrich Product Name KHOS-240S, 86112810 biological source human bone growth mode Adherent
List Price $436.00 Your Price $436.00
Sigma-Aldrich Product Name KHOS-240S, 86112810 biological source human bone growth mode Adherent karyotype 2n = 46, aneuploid morphology Epithelial products Not specified receptors Not specified - SIALUPENN (Additional S&H or Hazmat Fees may Apply)
NETA PART: SIALUPENN-86112810-DNA-5UG
MFG.PART: 86112810-DNA-5UG
UNSPSC: 41122409
Manufacturer: Sigma-Aldrich
Product Name
KHOS-240S, 86112810
biological source
human bone
growth mode
Adherent
karyotype
2n = 46, aneuploid
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
Related Categories
Cell Culture & Analysis
Cell Lines & Specialty Cell Culture
Mammalian Cell Lines
Molecular Biology & Functional Genomics
Cell Line Origin
Human Caucasian osteosarcoma
Cell Line Description
A revertant line of the KHOS/NP cell line (Sigma Catalogue number. 84102903). Established after overnight incubation at 40.5°C and then sub-cloned at 36°C. The line has similar properties to the parent HOS cell line. Cells are non-tumourigenic in nude mice and no longer possess a rescuable MSV genome.
application
Tumourigenicity, transformation, biochemical and virus studies: MSV
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 11
vWA: 18
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 11
vWA: 18
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
| SKU | SIALUPENN-86112810-DNA-5UG |
|---|---|
| Supplier Part Number | 86112810-DNA-5UG |
| UM | EA |
| UNSPSC | 41122409 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIALUPENN |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |