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Sigma-Aldrich Product Name G-401, 87042204 biological source human kidney growth mode Adherent
List Price $436.00 Your Price $436.00
Sigma-Aldrich Product Name G-401, 87042204 biological source human kidney growth mode Adherent karyotype 2n = 46, diploid morphology Epithelial-like products Not specified receptors Not specified - SIALUPENN (Additional S&H or Hazmat Fees may Apply)
NETA PART: SIALUPENN-87042204-DNA-5UG
MFG.PART: 87042204-DNA-5UG
UNSPSC: 41122409
Manufacturer: Sigma-Aldrich
Product Name
G-401, 87042204
biological source
human kidney
growth mode
Adherent
karyotype
2n = 46, diploid
morphology
Epithelial-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
Related Categories
Cell Culture & Analysis
Cell Lines & Specialty Cell Culture
Mammalian Cell Lines
Molecular Biology & Functional Genomics
Cell Line Origin
Human Caucasian rhabdoid tumour (formerly classified as Wilm′s tumour)
Cell Line Description
Derived from a tumour of a 3 month old male Caucasian. Highly transformed and grows in soft agar. Highly undifferentiated. G401 was originally described as a cell line derived from a Wilm?s tumour. Due to a change in the classification of such tumours, the cell line was examined by Garvin et al., 1993 and found to be more appropriately classified as derived from a rhabdoid tumour of the kidney.
Application
Transformation and tumourigenicity studies
DNA Profile
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,13
D13S317: 9,14
D16S539: 12
D5S818: 13
D7S820: 11,14
THO1: 8,9.3
TPOX: 8,11
vWA: 16
CSF1PO: 11,13
D13S317: 9,14
D16S539: 12
D5S818: 13
D7S820: 11,14
THO1: 8,9.3
TPOX: 8,11
vWA: 16
Culture Medium
McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Change medium every 4 days.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
| SKU | SIALUPENN-87042204-DNA-5UG |
|---|---|
| Supplier Part Number | 87042204-DNA-5UG |
| UM | EA |
| UNSPSC | 41122409 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIALUPENN |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |