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Sigma-Aldrich biological source human blood ethnicity French gender female growth mode Suspension
List Price
$436.00
Your Price
$436.00
Sigma-Aldrich biological source human blood ethnicity French gender female growth mode Suspension karyotype Not specified morphology Lymphoblastoid technique(s) cell culture | mammalian: suitable - SIALUPENN (Additional S&H or Hazmat Fees may Apply)
NETA PART:
SIALUPENN-88052045-DNA-5UG
MFG.PART:
88052045-DNA-5UG
UNSPSC:
41122409
Manufacturer:
Sigma-Aldrich
biological source
human blood
ethnicity
French
gender
female
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblastoid
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
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General description
Consanguineous: No
Cell Line Description
This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.
DNA Profile
A: 0216 0301
Cw: 0704 1502
B: 5101
HLA DRA: 0101 0102
HLA DRB1: 1104 1201
HLA DRB3: 202
HLA DQA1: 501
HLA DQB1: 301
HLA DPA1: 1
HLA DPB1: 02012 0402
Cw: 0704 1502
B: 5101
HLA DRA: 0101 0102
HLA DRB1: 1104 1201
HLA DRB3: 202
HLA DQA1: 501
HLA DQB1: 301
HLA DPA1: 1
HLA DPB1: 02012 0402
Serological Profile
HLA A: 2 3
HLA A ief: 2.3 3.2
HLA C: 7
HLA B: 51
HLA B ief: 51
Bw4/bw6: 4
HLA Dw: 5 DB6
HLA DR: 11 12
HLA Dw2: 25
HLA DR52: 52
HLA DQ: 7
HLA DP: 2 4
HLA A ief: 2.3 3.2
HLA C: 7
HLA B: 51
HLA B ief: 51
Bw4/bw6: 4
HLA Dw: 5 DB6
HLA DR: 11 12
HLA Dw2: 25
HLA DR52: 52
HLA DQ: 7
HLA DP: 2 4
Culture Medium
RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.
Subculture Routine
Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.
| SKU | SIALUPENN-88052045-DNA-5UG |
|---|---|
| Supplier Part Number | 88052045-DNA-5UG |
| UM | EA |
| UNSPSC | 41122409 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIALUPENN |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |