We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn about our cookie policy.
Sigma-Aldrich biological source human blood ethnicity Danish gender female growth mode Suspension
List Price $436.00 Your Price $436.00
Sigma-Aldrich biological source human blood ethnicity Danish gender female growth mode Suspension karyotype Not specified morphology Lymphoblastoid technique(s) cell culture | mammalian: suitable - SIALUPENN (Additional S&H or Hazmat Fees may Apply)
NETA PART: SIALUPENN-88052050-DNA-5UG
MFG.PART: 88052050-DNA-5UG
UNSPSC: 41122409
Manufacturer: Sigma-Aldrich
biological source
human blood
ethnicity
Danish
gender
female
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblastoid
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
Looking for similar products? Visit Product Comparison Guide
General description
Consanguineous: Yes
Homozygous: Yes
Cell Line Description
This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.
DNA Profile
A: 2902
Cw: 1601
B: 44031
HLA DRB1: 701
HLA DRB4: 101
HLA DQAP: 2.1
HLA DQA1: 201
HLA DQB1: 202
HLA DPA1: 1
HLA DPB1: 2012
Cw: 1601
B: 44031
HLA DRB1: 701
HLA DRB4: 101
HLA DQAP: 2.1
HLA DQA1: 201
HLA DQB1: 202
HLA DPA1: 1
HLA DPB1: 2012
Serological Profile
HLA A: 29
HLA A ief: 29.2
HLA B: 44
HLA B ief: 44.1
Bw4/bw6: 4
HLA Dw: 7
HLA DR: 7
HLA DR53: 53
HLA DQ: 2
HLA DP: 2
HLA A ief: 29.2
HLA B: 44
HLA B ief: 44.1
Bw4/bw6: 4
HLA Dw: 7
HLA DR: 7
HLA DR53: 53
HLA DQ: 2
HLA DP: 2
Culture Medium
RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.
Subculture Routine
Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.
| SKU | SIALUPENN-88052050-DNA-5UG |
|---|---|
| Supplier Part Number | 88052050-DNA-5UG |
| UM | EA |
| UNSPSC | 41122409 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIALUPENN |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |