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Sigma-Aldrich Product Name A2, 89030316 biological source hamster ovary growth mode Adherent karyoty
List Price $436.00 Your Price $436.00
Sigma-Aldrich Product Name A2, 89030316 biological source hamster ovary growth mode Adherent karyotype Not specified morphology Epithelial products Ornithine decarboxylase receptors Not specified - SIALUPENN (Additional S&H or Hazmat Fees may Apply)
NETA PART: SIALUPENN-89030316-DNA-5UG
MFG.PART: 89030316-DNA-5UG
UNSPSC: 41122409
Manufacturer: Sigma-Aldrich
Product Name
A2, 89030316
biological source
hamster ovary
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Ornithine decarboxylase
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Hamster Chinese ovary
Cell Line Description
Overproduces ornithine decarboxylase in response to polyamine deprivation.
Application
Enzymatic studies
Culture Medium
EMEM (EBSS) + 2mM Glutamine + Ham′s F12 (without Putrescine) (1:1) + 0.1% BSA.
Subculture Routine
Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000 to 1x10,000 cells/cm2 using cold PBS and 0.25% cold trypsin. Place flasks at 4°C for 5-10 minutes or until cells detach. Culture cells at 37°C; 5% CO2. Pre-soak flasks in porcine
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
| SKU | SIALUPENN-89030316-DNA-5UG |
|---|---|
| Supplier Part Number | 89030316-DNA-5UG |
| UM | EA |
| UNSPSC | 41122409 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIALUPENN |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |