We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn about our cookie policy.
Sigma-Aldrich Product Name H4II, 89042702 biological source rat skin growth mode Adherent karyotype
List Price $436.00 Your Price $436.00
Sigma-Aldrich Product Name H4II, 89042702 biological source rat skin growth mode Adherent karyotype 52 chromosomes morphology Epithelial products Albumin, transferrin, prothrombin receptors Not - SIALUPENN (Additional S&H or Hazmat Fees may Apply)
NETA PART: SIALUPENN-89042702-DNA-5UG
MFG.PART: 89042702-DNA-5UG
UNSPSC: 41122409
Manufacturer: Sigma-Aldrich
Product Name
H4II, 89042702
biological source
rat skin
growth mode
Adherent
karyotype
52 chromosomes
morphology
Epithelial
products
Albumin, transferrin, prothrombin
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
Related Categories
Cell Culture & Analysis
Cell Lines & Specialty Cell Culture
Mammalian Cell Lines
Molecular Biology & Functional Genomics
Cell Line Origin
Rat skin Hepatocellular carcinoma
Cell Line Description
From H-35 Reuber hepatoma induced by chemical carcinogenesis in an AxC rat . Tyrosine aminotransferase positive. Synthesises several serum proteins including albumin, transferrin and prothrombin. (Same as H4-11-E-C3 (Sigma Catalogue number. 85061112)).
Application
Gene expression analysis.
Culture Medium
Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
Subculture Routine
Split semi-confluent [60%-70%] cultures 1:100 i.e. seeding at 2x100 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Saturation density is reached at 15x1000,000 cells/10cm2 dish.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
| SKU | SIALUPENN-89042702-DNA-5UG |
|---|---|
| Supplier Part Number | 89042702-DNA-5UG |
| UM | EA |
| UNSPSC | 41122409 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIALUPENN |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |