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Sigma-Aldrich Product Name WI 38 Cell Line human, caucasian fibroblast-like fetal lung cell, 9002010
List Price $436.00 Your Price $436.00
Sigma-Aldrich Product Name WI 38 Cell Line human, caucasian fibroblast-like fetal lung cell, 90020107 biological source human lung (fetal) growth mode Adherent karyotype 2n = 46, diploid except at - SIALUPENN (Additional S&H or Hazmat Fees may Apply)
NETA PART: SIALUPENN-90020107-DNA-5UG
MFG.PART: 90020107-DNA-5UG
UNSPSC: 41122409
Manufacturer: Sigma-Aldrich
Product Name
WI 38 Cell Line human, caucasian fibroblast-like fetal lung cell, 90020107
biological source
human lung (fetal)
growth mode
Adherent
karyotype
2n = 46, diploid except at high passage number
morphology
Fibroblast-like
products
Collagen
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human Caucasian foetal lung
Cell Line Description
From normal embryonic lung of female. Have very broad human virus spectra, especially useful for isolation of rhinoviruses. Cells form multilayered membrane when held for long periods at 37°C with periodic pH adjustments. Used in preparation of human virus vaccines.
Application
From normal female embryonic lung (expect collagen production). These cells have a very broad human virus spectra. Particularly useful for the isolation of rhinovirus. Cells from multilatered membrane when incubated for long periods with periodic pH adjustments. Used in the preparation of human virus vaccines.
Subculture: Spit at 70-80% confluency, approx. 1:3 to 1:6, seed at 2-4x104 viable cells/cm2. Trypsinize using 0.25% solution, with or without EDTA, 37 °C and 5% CO2.
Virus studies (rhinoviruses), vaccine production
WI 38 human cell line has also been used to study the cytotoxicity of acrylic based resin compounds and silica nanoparticles.[1][2]
Biochem/physiol Actions
Finite lifespan. Approx. 50, +/- 10, population doublings. Normal doubling time is 24 hours.
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 10
D7S820: 9,11
THO1: 8,9.3
TPOX: 8
vWA: 19,20
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 10
D7S820: 9,11
THO1: 8,9.3
TPOX: 8
vWA: 19,20
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Finite lifespan, 50 plus or minus 10 population doublings expected - doubling time 24h.
Analysis Note
Recommended culture medium: EMEM (EBSS) + 2 mM L-Glutamine + 1% Non-essential amino acids (NEAA) + 15% FBS
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
| SKU | SIALUPENN-90020107-DNA-5UG |
|---|---|
| Supplier Part Number | 90020107-DNA-5UG |
| UM | EA |
| UNSPSC | 41122409 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIALUPENN |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |