Sigma-Aldrich Product Name Cri du Chat, 90102533 biological source human skin growth mode Adherent

Human Caucasian skin deletion in chromosome 5

Derived from the skin of an adult female Caucasian with Cri du Chat syndrome. The cells have a deletion in chromosome 5 and have a finite lifespan; up to 35 population doublings expected.

Chromosome studies

McCoy′s 5a + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Cells should not be centrifuged.

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