Sigma-Aldrich Product Name HG261, 90112603 biological source human skin growth mode Adherent karyoty

Human Caucasian skin Fanconi′s anaemia

From 6 year old Caucasian male with Faconi′s anaemia which is associated with high incidence of leukaemia. Chromosomes show a tendency to develop spontaneous abnormalities including breaks and complex rearrangement. Have a higher proportion of transformed colonies with SV40 compared with normal diploid cells.

Chromosome and virus studies: SV40

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Limited lifespan; up to 35 passages possible.

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