Sigma-Aldrich Product Name PC-3 Cell Line human, 90112714, Caucasian prostate adenocarcinoma biologi

Human Caucasian prostate adenocarcinoma

Established from a grade 4 prostatic adenocarcinoma from a 62 year old male Caucasian. The cells grow in agar and produce tumours in nude mice. Exhibit low acid phosphatase and testosterone-5-α reductase activity. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

PC-3 cell line has been used to study the antiproliferative effects of F. mughlea Pesmen on cancer cells.[1] It has also been used to study the effects of palmitoylcarnitine (palcar) on the gene expression and pro-inflammatory pathways in prostate cancer.[2]

Tumourigenicity studies

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 11
D5S818: 13
D7S820: 8,11
THO1: 6,7
TPOX: 8,9
vWA: 17

Coons Modified Ham’s F12 + 2mM Glutamine + 7% Foetal Bovine Serum (FBS).OR Kaign’s modified Ham’s F12 + 45mg/L ascorbic acid + 18 mg/L Inositol + 2mM Glutamine + 7% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Medium change every 5 days. The initial subculture interval after cells are thawed may be longer than 7-9 days.

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