Sigma-Aldrich Product Name SK-HEP-1, 91091816 biological source human liver growth mode Adherent

Human liver adenocarcinoma

Derived from an ascites sample from a 52 year old male suffering from adenocarcinoma of the liver. The cells have now been shown to be endothelial in origin (In Vitro 1992;28A:136). The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 8,12
D16S539: 12
D5S818: 10,13
D7S820: 8,11
THO1: 7,9
TPOX: 9
vWA: 14,17

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

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