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Sigma-Aldrich Product Name HT2 Clone A5E, 92021401 biological source mouse blood growth mode Suspens
List Price $436.00 Your Price $436.00
Sigma-Aldrich Product Name HT2 Clone A5E, 92021401 biological source mouse blood growth mode Suspension karyotype Not specified morphology Lymphoblast products Not specified receptors Interleukin 2 - SIALUPENN (Additional S&H or Hazmat Fees may Apply)
NETA PART: SIALUPENN-92021401-DNA-5UG
MFG.PART: 92021401-DNA-5UG
UNSPSC: 41122409
Manufacturer: Sigma-Aldrich
Product Name
HT2 Clone A5E, 92021401
biological source
mouse blood
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblast
products
Not specified
receptors
Interleukin 2 (IL-2), Interleukin 4 (IL-4)
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Culture & Analysis
Cell Lines & Specialty Cell Culture
Mammalian Cell Lines
Molecular Biology & Functional Genomics
Cell Line Origin
Mouse helper T cell
Cell Line Description
Derived in 1979 from BALB/c spleen cells activated by sheep erythrocytes in the presence of IL-2, the A5E clone was selected to provide a homogenous population that is highly sensitive to IL-2. This cell line expresses Thy-1 and Lyt markers in addition to high affinity IL-2 receptors. Substantial proliferation is seen in the presence of IL-4 (GM-CSF).
Application
IL-2 assays
Culture Medium
RPMI 1640 + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 100-200 IU/ml IL-2 + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. When starting from a frozen ampoule, add thawed cells to a conical based centrifuge tube e.g. 15ml tube. Slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
| SKU | SIALUPENN-92021401-DNA-5UG |
|---|---|
| Supplier Part Number | 92021401-DNA-5UG |
| UM | EA |
| UNSPSC | 41122409 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIALUPENN |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |