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Sigma-Aldrich Product Name ND8/34 Cell Line from mouse, 92090904 biological source Mouse x Rat
List Price $1,060.00 Your Price $1,060.00
Sigma-Aldrich Product Name ND8/34 Cell Line from mouse, 92090904 biological source Mouse x Rat Hybridoma nerve description Mouse neuroblastoma x Rat neurone hybrid growth mode Adherent karyotype Not - SIALUPENN (Additional S&H or Hazmat Fees may Apply)
NETA PART: SIALUPENN-92090904-1VL
MFG.PART: 92090904-1VL
UNSPSC: 41122409
Manufacturer: Sigma-Aldrich
Product Name
ND8/34 Cell Line from mouse, 92090904
biological source
Mouse x Rat Hybridoma nerve
description
Mouse neuroblastoma x Rat neurone hybrid
growth mode
Adherent
karyotype
Not specified
morphology
Neuronal
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
Cell Line Origin
Mouse neuroblastoma x Rat neurone hybrid
Cell Line Description
The hybrid ND8/34 is a subclone of ND8 which was derived after fusion of primary neonatal rat dorsal-root-ganglion (DRG) neurons with N18Tg2, a mouse neuroblastoma (C1300)-derived azaguanine-resistant line. HAT sensitive hybrids were selected and cloned by limiting dilution. ND8/34 are electrically excitable to the chemical activator of nociceptive sensory neurons, bradykinin.
Application
Study of neuronal differentiation.
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 by either aspirating or gently tapping the cells loose; 5% CO2; 37°C. Cells are lightly attached therefore use of trypsin is unnecessary.
Other Notes
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| SKU | SIALUPENN-92090904-1VL |
|---|---|
| Supplier Part Number | 92090904-1VL |
| UM | EA |
| UNSPSC | 41122409 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIALUPENN |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |