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Sigma-Aldrich Product Name ND-C, 92090913 biological source mouse nerve growth mode Adherent karyoty
List Price $436.00 Your Price $436.00
Sigma-Aldrich Product Name ND-C, 92090913 biological source mouse nerve growth mode Adherent karyotype Not specified morphology Neuronal products Not specified receptors Not specified technique(s) - SIALUPENN (Additional S&H or Hazmat Fees may Apply)
NETA PART: SIALUPENN-92090913-DNA-5UG
MFG.PART: 92090913-DNA-5UG
UNSPSC: 41122409
Manufacturer: Sigma-Aldrich
Product Name
ND-C, 92090913
biological source
mouse nerve
growth mode
Adherent
karyotype
Not specified
morphology
Neuronal
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
Related Categories
Cell Culture & Analysis
Cell Lines & Specialty Cell Culture
Mammalian Cell Lines
Molecular Biology & Functional Genomics
Cell Line Origin
Mouse neuroblastoma x Rat neurone hybrid
Cell Line Description
ND-C hybrid cells were derived after fusion of primary neonatal-rat dorsal-root-gauglion (DRG) neurons with N18Tg2, a mouse neuroblastoma (C1300) derived azaguanine resistant line. HAT sensitive hybrids were selected and cloned by limiting dilution. The cells express rat Thy 1.1 marker and the sensory neuropeptide substance P. The cell line has been mycoplasma eradicated at ECACC.
Application
Study of neuronal differentiation, cell adhesion and expression of neuronal proteins.
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3 x 10,000 cells/cm2; aspirate or gently tap cells loose, use of trypsin not necessary; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
| SKU | SIALUPENN-92090913-DNA-5UG |
|---|---|
| Supplier Part Number | 92090913-DNA-5UG |
| UM | EA |
| UNSPSC | 41122409 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIALUPENN |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |