Sigma-Aldrich Product Name BE10-Intermediate, 93040119 biological source rat brain growth mode Adher

Rat, BDIX, foetal brain, pre-malignant

Produced by passaging the latent period cell line BE10-Early (Sigma Catalogue number. 93040118) beyond passage 17. The cells possess elevated levels of tissue plasminogen activator activity, are not tumourigenic in BDIX rats and do not form colonies in agar. Do not be use beyond passage 40 as cell properties alter. The BE10 culture was originally derived 2 days after a single dose of ethylnitrosourea to pregnant rats. The control culture BE11 was derived 2 days after exposure to buffer.

Study of changes in the latent period of carcinogenesis

DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x100,000 cells/cm² using 0.25% trypsin/EDTA; 10-15% CO₂; 37°C.

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