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Sigma-Aldrich Product Name RC-4B/C1, 94020810 biological source rat brain growth mode Adherent
List Price
$436.00
Your Price
$436.00
Sigma-Aldrich Product Name RC-4B/C1, 94020810 biological source rat brain growth mode Adherent karyotype Not specified morphology Epithelial-like products Gonadatrophs receptors - SIALUPENN (Additional S&H or Hazmat Fees may Apply)
NETA PART:
SIALUPENN-94020810-DNA-5UG
MFG.PART:
94020810-DNA-5UG
UNSPSC:
41122409
Manufacturer:
Sigma-Aldrich
Product Name
RC-4B/C1, 94020810
biological source
rat brain
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial-like
products
Gonadatrophs
receptors
Gonadotrophin-releasing hormone receptors
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
Related Categories
Cell Culture & Analysis
Cell Lines & Specialty Cell Culture
Mammalian Cell Lines
Molecular Biology & Functional Genomics
Cell Line Origin
Rat pituitary adenoma
Cell Line Description
RC-4B/C1 is a clone of the parent RC-4B/C which was established from a 3 year-old CAI/FMaixF344/FMai(F1) male rat pituitary adenoma. Staining studies have shown the cell line contains many bi-hormonal cells with FHSß, LHß and prolactin. Electron microscopy shows the appearance of endogenous C-type rat retroviral particles.
Application
Study of gonadotrophic functions of pituitary gland and regulation of pituitary gonadotrophin gene expression.
Culture Medium
DMEM:EMEM (EBSS) (1:1) + 2mM Glutamine + 0.2mg/ml BSA + 0.5% Non Essential Amino Acids (NEAA) + 5% Nu-Serum (Collab)+ 2ng/ml EGF.
Subculture Routine
Split sub-confluent cultures (70-80%) 1:6 i.e. seeding at 2x10,000 cells/cm2 using 0.25% trypsin/EDTA; 10% CO2; 34°C. Saturation density is reached at 1x 100,000 cells/cm2. Cells may attach lightly and so handling should be kept to a minimum. Care shoul
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
| SKU | SIALUPENN-94020810-DNA-5UG |
|---|---|
| Supplier Part Number | 94020810-DNA-5UG |
| UM | EA |
| UNSPSC | 41122409 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIALUPENN |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |