Sigma-Aldrich TISSUEFAB(R) TYRAMINE FUNCTIONALIZED PO&

Rethink your bioinks with TissueFab^®About the material: These TissueFab^® tyramine functionalized poly(amino acid) is a xeno-free (animal origin free), biodegradable and biocompatible polymer that been developed and tested for hydrogel synthesis for tissue engineering and other biomedical applications.


Printing and crosslinking parameters:

• Compatible for cell encapsulation and hydrogel formation
• Crosslinked via a ruthenium photoinitiatior system

The TissueFab^® tyramine functionalized poly(amino acid) is characterized by its biocompatibility and biodegradability, making it well-suited for a broad spectrum of applications in the biomedical field. This poly(amino acid) is synthesized through the ring opening of polysuccinimide using a nucleophilic reagent. The presence of amide linkages in these polymers results in peptide-like structures that are prone to cleavage and degradation in the body, leading to the formation of amino acids or small peptides. The tyramine functional group within the polymer provides sites for further chemical modification or conjugation with bioactive molecules, such as drugs, peptides, and targeting ligands. For example, the free amine group on tyramine can undergo acylation with acid chlorides or anhydrides to form amide derivatives. Additionally, tyramine can participate in enzymatic or chemical crosslinking reactions to form covalent bonds between tyramine molecules or with other biomolecules. Furthermore, this tyramine functionalized poly(amino acid) can be photo-oxidized by the Ruthenium photoinitiator kit (Cat#916881) into reactive intermediates, which can then crosslink with proteins or adjacent tyramine to form a cell compatible hydrogel.
More information and a step by step protocol can be found under the "More Documents" section of this page.

The TissueFab^® tyramine functionalized poly(amino acid) xeno-free polymer is:

• Biocompatible and biodegradable precursor for hydrogel and bioink development
• Suitable for a wide range of biomedical applications, including 3D cell culture, tissue engineering, in vitro drug screening, and regenerative medicine research
• Enables the development of xeno-free polymers for applications in advanced cell culture and tissue engineering
• Ideal for creating biocompatible materials that support cell growth and tissue regeneration in a xenogeneic-free environment

This TissueFab^® hydrogel and bioink precursor has been developed and tested to meet strict criteria to best suit researchers′ needs. This includes:

• Xeno-free, animal-origin free
• Tunable
• Cell viability
• Biocompatibility and biodegradability
• Mechanical and rheological properties
• Crosslinkability

TISSUEFAB is a registered trademark of Merck KGaA, Darmstadt, Germany