Sigma-Aldrich Product Name M1WT3, 940822109 biological source hamster ovary growth mode Adherent

Hamster Chinese ovary (transfected with rat m1 mAChR encoding gene).

Derived in 1990 by transfecting Chinese Hamster ovary CHO-K1 cells with the gene encoding rat m1 muscarinic acetylcholine receptor (mAChR) in the mammalian expression vector, pSVL. These cells were then co-transfected with pMSVNeo and selected for growth in selection medium containing 500μg/ml G418. The cells are reported to express 800 fmol of receptor/mg of membrane protein.

This cell line can be used to study m1 mAChR in cells devoid of other muscarinic receptor subtypes.

Ham′s F12 + 2mM Glutamine + 50-100μg/ml G418 + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

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