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Sigma-Aldrich Product Name SK-N-F1, 94092304 biological source human nerve (bone marrow metastasis)
List Price $917.00 Your Price $917.00
Sigma-Aldrich Product Name SK-N-F1, 94092304 biological source human nerve (bone marrow metastasis) growth mode Adherent/aggregates in suspension karyotype Aneuploid morphology Epithelial-like - SIALUPENN (Additional S&H or Hazmat Fees may Apply)
NETA PART: SIALUPENN-94092304-1VL
MFG.PART: 94092304-1VL
UNSPSC: 41122409
Manufacturer: Sigma-Aldrich
Product Name
SK-N-F1, 94092304
biological source
human nerve (bone marrow metastasis)
growth mode
Adherent/aggregates in suspension
karyotype
Aneuploid
morphology
Epithelial-like
products
Not specified
receptors
Transferrin receptor
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
metastasis
shipped in
dry ice
Related Categories
Cell Culture & Analysis
Cell Lines & Specialty Cell Culture
Mammalian Cell Lines
Molecular Biology & Functional Genomics
Cell Line Origin
Human neuroblastoma
Cell Line Description
SK-N-F1 has been obtained from a bone marrow metastasis of a male patient with neuroblastoma. The poorly differentiated and slow growing cells secrete different neuroblastoma specific peptides and synthesise choline acetyltransferase. Reactivity of anti-leukaemia-associated and anti-foetal brain-associated monoclonal antibodies could be shown. Epithelial-like and aggregate-forming populations.
Application
Carcinogenesis studies, cytotoxicity assays.
DNA Profile
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11,14
D16S539: 11,12
D5S818: 12,13
D7S820: 8,9
THO1: 6,9.3
TPOX: 8,9
vWA: 16,17
CSF1PO: 10,12
D13S317: 11,14
D16S539: 11,12
D5S818: 12,13
D7S820: 8,9
THO1: 6,9.3
TPOX: 8,9
vWA: 16,17
Culture Medium
DMEM + 4mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 2-4x10,000 cells/ml using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Clumped cells have tendency to float.
Other Notes
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| SKU | SIALUPENN-94092304-1VL |
|---|---|
| Supplier Part Number | 94092304-1VL |
| UM | EA |
| UNSPSC | 41122409 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIALUPENN |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |