Sigma-Aldrich Product Name BW1J, 94101901 biological source mouse skin growth mode Adherent karyotyp

Mouse hepatoma

A mouse hepatoma isolated from the BW7756 transplantable hepatoma carried in C57 leaden/J mice (see reference: Differentiation). Will synthesise in appreciable amounts only hepatic proteins that appear early in foetal development. The majority of cells contain at least two and often several nucleoli, fat droplets are rare. A mixture of BW1-J and Fao cells were treated with inactive Sendai Virus to produce hybrids at 1 hybrid colony/50 BW1-J cells.

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS)

Split semi-confluent cultures (70%) 1:50 i.e. seeding at 2x1000 - 5x1000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Media change three times a week.

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