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Sigma-Aldrich biological source Horse x Mouse/Horse Hybridoma ImmunoDon Horse peripheral lymphocytes
List Price $436.00 Your Price $436.00
Sigma-Aldrich biological source Horse x Mouse/Horse Hybridoma ImmunoDon Horse peripheral lymphocytes Myeloma NS0 Reactivity Application: Fusion partner for hybridoma production technique(s) cell - SIALUPENN (Additional S&H or Hazmat Fees may Apply)
NETA PART: SIALUPENN-94120825-DNA-5UG
MFG.PART: 94120825-DNA-5UG
UNSPSC: 41122409
Manufacturer: Sigma-Aldrich
biological source
Horse x Mouse/Horse Hybridoma
ImmunoDon
Horse peripheral lymphocytes
Myeloma
NS0
Reactivity
Application: Fusion partner for hybridoma production
technique(s)
cell culture | mammalian: suitable
isotype
Not specified
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Horse peripheral blood lymphocytes x Mouse NS0
Cell Line Description
The heterohybridoma AFRC 17D1 was obtained by fusion of lymphocytes from ponies immunised with the influenza A equine 2 virus (isolateA/Equine/Newmarket/79 (H3N8)) with mouse myeloma NS0 cells. The hybridoma was made aminopterin-sensitive by selective growth in 8-azaguanine. This non-Ig-secreting cell line was used as fusion partner for the establishment of equine-mouse heterohybidomas.
Immunogen
Influenza A equine 2 virus
Application
Fusion partner for hybridoma production
Culture Medium
RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 5% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
| SKU | SIALUPENN-94120825-DNA-5UG |
|---|---|
| Supplier Part Number | 94120825-DNA-5UG |
| UM | EA |
| UNSPSC | 41122409 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIALUPENN |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |