Takara IVTpro T7 mRNA Synthesis Kit (low dsRNA). 20 Rxns

The Takara IVTpro mRNA Synthesis Kit enables the production of large amounts of mRNA through in vitro transcription, synthesizing up to 200 μg or more of mRNA per 20 μl reaction.

The kit includes a highly efficient T7 RNA polymerase, separate NTPs for easy optimization or replacement with modified nucleosides, DNase I to digest the template DNA after the in vitro transcription reaction, a lithium chloride (LiCl) solution to purify the synthesized mRNA for downstream applications, and a Positive Control Template (FLuc) plasmid containing the T7 promoter, transcription initiation sequence (AGG), 5- and 3-UTRs, FLuc CDS, and poly(A) sequence.

Cat. # 6134 has anEnzyme Mix containing PrimeCap T7 RNA Polymerase (low dsRNA) for reduced dsRNA production.