Revvity HTFF Human Phospho-DAP12 (TYR91) Detection Kit, 500 Assay Points

This HTRF cell-based assay provides a convenient and accurate method for detecting phosphorylated DAP12 at Tyr91. DAP12 (DNAX-Activation Protein 12), also known as TYROBP (TYRO protein tyrosine kinase-binding protein), is a transmembrane signaling adaptor protein that plays a crucial role in innate immune responses and neuroinflammation. DAP12 is widely expressed in various immune cells, including natural killer cells, monocytes, macrophages, and microglia, the resident immune cells of the central nervous system. In the context of neuroinflammation, DAP12 is particularly important due to its association with the TREM2 (Triggering Receptor Expressed on Myeloid cells 2) receptor. The DAP12-TREM2 signaling axis is a key regulator of microglial function, influencing processes such as phagocytosis, inflammatory responses, and lipid metabolism. By enabling the detection of phosphorylated DAP12 at Tyr91, this assay provides researchers with a valuable tool for investigating microglial activation, neuroinflammatory processes, and the potential effects of therapeutic interventions targeting the DAP12-TREM2 pathway.