Takara Lenti-X™ T-Cell Transduction Sponge, 12 Rxns

The Lenti-X T-Cell Transduction Sponge expedites and streamlines lentiviral transduction of T cells, bypassing the need for prior activation or spinoculation with chemical enhancers. This innovative approach employs a macroporous alginate matrix infused with an optimized blend of rhIL-2 (100 IU) and anti-human CD3 & CD28 antibodies. This combination with the sponge not only activates T cells but also enhances lentiviral transduction by fostering the co-localization of activated T cells and lentivirus particles.

Visual overview of the Lenti-X T-Cell Transduction Sponge workflow.

Lenti-X T-Cell Transduction Sponge.Panel A.Sponges (12) in the crush-resistant blister packaging and magnified view of the macroporous cell structure of the alginate.Panel B.Illustrated visualization of colocalization of T cells and virus promoted by the sponge structure.

Effective T-cell transduction with the Lenti-X T-Cell Transduction Sponge. 4 x 106human primary T cells were mixed with a lentivirus expressing ZsGreen1 at the indicated MOI and added to the Lenti-X T-Cell Transduction Sponge ("T-cell sponge"). Spinoculated samples were activated with CD3/CD28 ImmunoCult reagent for two days, then exposed to the lentivirus with polybrene (8 µg/mL) and centrifuged at 1,500gfor 90 min ("Spinoculation"). Transduction efficiency was assessed by FACS analysis for ZsGreen1 expression at the indicated time points. Displayed percentages show GFP+ expression at seven days.

High T-cell viability after transduction with the Lenti-X T-Cell Transduction Sponge. 4 x 106human primary T cells were mixed with a lentivirus expressing ZsGreen1 at the indicated infection MOIs and added to the Lenti-X T-cell Transduction Sponge ("T-cell sponge"). Spinoculated samples were activated with CD3/CD28 ImmunoCult reagent for two days, then exposed to the lentivirus with polybrene (8 µg/mL) and centrifuged at 1,500gfor 90 min ("Spinoculation"). Viability was assessed at indicated timepoints post-transduction by 7-AAD staining and FACS analysis.

Efficient T-cell activation with the Lenti-X T-Cell Transduction Sponge. 4 x 106human primary T cells were mixed with a lentivirus expressing ZsGreen1 at the indicated multiplicities of infection (MOIs) and added to the Lenti-X T-cell Transduction Sponge ("T-cell sponge") or treated with CD3/CD28 Immunocult reagent ("Conjugate"). Unstimulated cells were used as a control ("Unstim"). After 48 hr, treated cells were assayed for CD69 expression by FACS analysis.