Takara BcaBEST™ Isothermal FluorDetect Kit (DNA/RNA), 100 Rxns

TheBcaBEST Isothermal FluorDetect Kit (DNA/RNA) allows for rapid detection of DNA or RNA targets via LAMP or one-step RT-LAMP. The kit includesBcaBEST Isothermal Mix (2X) and TB Green Solution (300X). Cat. # RR385A contains enough reagents for 100 reactions.

BcaBEST Isothermal FluorDetect Kit (DNA/RNA) achieved rapid detection of DNA and RNA samples.BcaBEST Isothermal FluorDetect Kit (DNA/RNA) was used to amplify DNA and RNA samples (template amount = 1, 10, 100, or 1,000 pg or 5, 50, 500, or 5,000 copies) at n = 2. The time to REACH a constant fluorescence intensity was measured. For these samples, detection was generally achieved within 20 min. The "1/2" in the figure indicates that one of the n = 2 was not detected within 30 min. For the NTC (no-template control), no amplification within 30 min was observed except for one NTC fororf-134. RNA samples: HL-60 total RNA (ACTBandALAS1) and SARS-CoV-2 RNA controls (Twist Bioscience). DNA samples:E. coligenomic DNA (fecA) and lambda phage genomic DNA (orf-134).

BcaBEST Isothermal FluorDetect Kit (DNA/RNA) has a faster detection time compared to other companies’ reagents using total RNA input.BcaBEST Isothermal FluorDetect Kit (DNA/RNA) and fluorescent LAMP detection reagents from various companies were used to amplify HL-60 total RNA (ACTB; template amount 1, 10, 100, or 1,000 pg) at n = 2. The time to REACH a certain fluorescence intensity was measured. TheBcaBEST Isothermal FluorDetect Kit (DNA/RNA) was capable of detection within 15 min.

BcaBEST Isothermal FluorDetect Kit (DNA/RNA) has a slightly faster detection time compared to other companies’ reagents using nasopharyngeal fluid and urine inputs.BcaBEST Isothermal FluorDetect Kit (DNA/RNA) and fluorescent LAMP detection reagents from various companies were used to detect SARS CoV-2 (noninfectious; 1,000 copies/rxn) spiked into nasopharyngeal fluid (amount = 0, 0.1, or 1 μl/rxn) at n = 2,Chlamydia trachomatis(noninfectious; 180 copies/rxn) spiked into urine (amount = 0, 2.25, or 4.5 μl/rxn) at n = 3, andNeisseria gonorrhoeae(noninfectious; 180 copies/rxn) spiked into urine (amount = 0, 2.25, or 4.5 μl/rxn) at n = 3. The spiked samples were treated with Proteinase K and then heat inactivated before the assay. The time to REACH a certain fluorescence intensity was measured.

BcaBEST Isothermal FluorDetect Kit (DNA/RNA) is compatible with Q-Probe detection.BcaBEST Isothermal FluorDetect Kit (DNA/RNA) was used to amplifyChlamydia trachomatiscontrol DNA (template amount = 1,000 or 200 copies/rxn at n = 2 and 0 copies/rxn at n = 6) andNeisseria gonorrhoeaecontrol DNA (template amount = 1,000 or 200 copies/rxn at n = 2 and 0 copies/rxn at n = 6). The time for the fluorescence of TB Green (intercalator) or Q-Probe to REACH the threshold were measured. Q-Probe hybridizes with amplified LAMP products, indicating decreasing fluorescence. The Q-Probe exhibits the same detection time as the intercalator but demonstrates more specificity in this assay.