Revvity AlphaLISA SureFire Ultra Human and Mouse Cleaved Caspase-3 (Asp175) Detection Kit, 50,000 Assay Points

Cleaved Caspase-3 (Asp175) represents the activated form of Caspase-3, a central executioner protease in the intrinsic and extrinsic apoptosis pathways. Upon cleavage at Asp175 by initiator caspases such as Caspase-8 or Caspase-9, active Caspase-3 induces proteolytic cleavage of key cellular substrates, leading to DNA fragmentation, cytoskeletal collapse, and eventual cell death. Cleaved Caspase-3 is widely recognized as a definitive marker of apoptosis and is commonly used to assess therapeutic efficacy in cancer, neurodegeneration, and tissue injury models. Dysregulation of Caspase-3 activation contributes to disease states characterized by impaired cell death or excessive tissue damage.

The AlphaLISA SureFire Ultra Human and Mouse Cleaved Caspase-3 (Asp175) Detection Kit is a sandwich immunoassay for the quantitative detection of cleaved Caspase-3 (Asp175) in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

AlphaLISA SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies