Revvity AlphaLISA SureFire Ultra Human Total DDR1 Detection Kit, 50,000 Assay Points

Discoidin Domain Receptor 1 (DDR1) is a receptor tyrosine kinase activated by collagen in the extracellular matrix. DDR1 modulates cell adhesion, migration, and matrix remodeling, playing a key role in development, tissue repair, and fibrosis. Its activation leads to downstream signaling through MAPK, PI3K/AKT, and NF-κB pathways. Overexpression and mutation of DDR1 have been implicated in various cancers, including breast, lung, and pancreatic cancer, where it contributes to tumor invasion, metastasis, and resistance to therapy. DDR1 is also associated with fibrotic diseases and has emerged as a promising target for anti-fibrotic and anti-cancer therapies.

The AlphaLISA SureFire Ultra Human Total DDR1 Detection Kit is a sandwich immunoassay for the quantitative detection of total DDR1 in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

AlphaLISA SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies