Revvity AlphaLISA SureFire Ultra Human and Mouse Total B-Raf Detection Kit, 50,000 Assay Points

B-Raf is a serine/threonine-protein kinase and a member of the RAF kinase family, acting downstream of RAS in the MAPK/ERK signaling pathway. It transmits mitogenic signals from the cell membrane to the nucleus, thereby regulating cellular processes such as proliferation, differentiation, and survival. The BRAF V600E mutation results in constitutive kinase activation and is a common driver in melanomas, colorectal cancer, and thyroid carcinoma. Targeted inhibitors against mutant BRAF have demonstrated clinical efficacy, though resistance often develops via MAPK reactivation or bypass mechanisms. Ongoing research explores combination therapies to prolong response and overcome resistance.

The AlphaLISA SureFire Ultra Human and Mouse Total B-Raf Detection Kit is a sandwich immunoassay for the quantitative detection of total B-Raf in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

AlphaLISA SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies