Revvity AlphaLISA SureFire Ultra Human and Mouse Total ARID1A Detection Kit, 50,000 Assay Points

AT-rich interactive domain-containing protein 1A (ARID1A) is a critical component of the SWI/SNF chromatin remodeling complex, which regulates gene expression by modulating chromatin structure and accessibility. ARID1A plays essential roles in transcriptional regulation, DNA damage repair, and the maintenance of genomic stability. It acts as a tumor suppressor, with frequent inactivating mutations observed in a variety of cancers, including ovarian clear cell carcinoma, endometrial cancer, and gastric cancer. Loss of ARID1A disrupts chromatin remodeling, leading to altered transcriptional programs, impaired DNA damage responses, and increased sensitivity to epigenetic and DNA repair inhibitors. Notably, synthetic lethality with EZH2 and ATR inhibitors has emerged as a promising therapeutic strategy, positioning ARID1A as a key biomarker and vulnerability in precision oncology.

The AlphaLISA SureFire Ultra Human and Mouse Total ARID1A Detection Kit is a sandwich immunoassay for the quantitative detection of total ARID1A in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

AlphaLISA SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies