Revvity AlphaLISA SureFire Ultra Human Total TNFAIP3 Detection Kit, 50,000 Assay Points

Tumor necrosis factor alpha-induced protein 3 (TNFAIP3), also known as A20, is a ubiquitin-editing enzyme that negatively regulates NF-κB signaling and inflammation. A20 exhibits both deubiquitinase and E3 ligase activities, allowing it to disassemble K63-linked polyubiquitin chains and add K48-linked chains for proteasomal degradation. This dual function is essential for maintaining immune homeostasis and preventing excessive inflammation. Loss-of-function mutations or reduced expression of A20 are associated with autoimmune diseases, lymphomas, and inflammatory conditions. A20 is a key checkpoint in immune signaling and a potential target for modulating chronic inflammation and enhancing anti-tumor responses.

The AlphaLISA SureFire Ultra Human Total TNFAIP3 Detection Kit is a sandwich immunoassay for the quantitative detection of total TNFAIP3 in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

AlphaLISA SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies