Revvity AlphaLISA SureFire Ultra Human and Mouse Total PDGFRα Detection Kit, 50,000 Assay Points

Platelet-Derived Growth Factor Receptor Alpha (PDGFRα) is a receptor tyrosine kinase involved in embryonic development, tissue repair, and angiogenesis. It binds PDGF-AA, -BB, and -CC ligands, activating downstream signaling pathways such as PI3K/AKT, MAPK, and STATs to promote proliferation, survival, and migration. PDGFRα mutations, amplifications, or fusions are oncogenic drivers in gastrointestinal stromal tumors (GISTs), gliomas, and dermatofibrosarcoma protuberans. Aberrant PDGFRα signaling is also implicated in fibrotic diseases and vascular disorders. Targeted therapies against PDGFRα have shown efficacy in PDGFR-driven tumors, and ongoing efforts aim to expand therapeutic targeting across disease contexts.

The AlphaLISA SureFire Ultra Human and Mouse Total PDGFRα Detection Kit is a sandwich immunoassay for the quantitative detection of total PDGFRα in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

AlphaLISA SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies