Revvity PhenoVue DNA Damage Response Staining Kit (γ-H2AX, 53 BP1), 1x384

Our ready-to-use PhenoVue DNA damage staining kit offers a reliable and straightforward solution for visualizing key markers of DNA damage and repair:

• γ-H2AX (Phospho S139 H2AX) - an early marker of double-strand breaks (DSBs), detected as distinct nuclear foci.
• 53BP1 - recruited to DSB sites and involved in non-homologous end joining (NHEJ) repair, forming punctate foci.
• Nucleus morphology - analyzed using PhenoLOGIC™ (in Harmony™ image analysis software) to assess late apoptosis through cell shrinkage and DNA fragmentation.

For enhanced flexibility, up to two additional biological markers such as PARP, ATM, or CHK can be included using the PhenoVue Fluor 555 and 400LS channels, allowing for deeper insights into DNA damage response (DDR) mechanisms.

The PhenoVue DNA Damage Staining Kit supports multiple drug discovery applications including:

• Oncology - allows the study of DDR-targeting therapeutics (e.g. PARP, ATM, ATR inhibitors), identification of synthetic lethal interactions (e.g. BRCA mutations), and assessment of genotoxic and cytotoxic effects.
• Genotoxicity and carcinogenicity - to detect DNA-damaging effects in lead compounds and help predict long-term carcinogenicity risks.
• Neurodegeneration and aging research - to investigate genomic instability and screening neuroprotective compounds.

Kit components and quantity

• PhenoVue Hoechst 33342 Nuclear Stain, 1 vial of 70 μL (500x)
• PhenoVue anti-Phospho H2AX (S139) - mouse IgG1 Antibody, 1 vial of 100 μL (100x)
• PhenoVue anti-53BP1 - rat IgG2a Antibody, 1 vial of 100 μL (100x)
• PhenoVue Fluor 488 - Goat anti-Mouse antibody Highly Cross Adsorbed, 1 vial of 100 μL (100x)
• PhenoVue Fluor 647 - Goat anti-Rat antibody Highly Cross Adsorbed, 1 vial of 100 μL (100x)
• PhenoVue Paraformaldehyde, 4% Solution, 1 vial of 25 mL (1x)
• PhenoVue Permeabilization, 0.5% Triton X-100 Solution, 1 vial of 25 mL (5x)
• PhenoVue dye diluent A, 1 vial of 8 mL (5x)