Sigma-Aldrich Tbe Buffer 10 X Ph 8.3 Tris-Borate-Edta

Tris, borate, and ethylenediaminetetraacetic (TBE) buffer is routinely used to conduct DNA and RNA agarose gel electrophoresis. It comprises a weak acid in neutral and anionic forms (e.g., COOH and COO^- species) and a weak base, Tris, which is either in neutral or cationic forms (Tris-NH₂ and Tris-NH₃⁺). These ions buffer the pH, maintain a low conductivity medium, and transfer the electrical current. Ethylenediaminetetraacetic (EDTA) is used to chelate Mg²⁺ ions and inactivate the potential DNA nucleases. TBE is also used to perform a good resolution of DNA fragments that slightly enhances the separation of smaller fragments.[1] It also protects nucleic acids from enzymatic degradation.[2]

Appearance (colour): almost colourless
Appearance (description): almost clear
pH-value: 8.2 - 8.4