Sigma-Aldrich Protein Delivery System Human Cxcl8 P&

Protein delivery system, Human CXCL8, PODS contains the polyhedrin protein co-crystalized with Human mature IL-8 (amino acids 23-99) which lacks the signal peptide and MDNCFα fragment. IL-8, also known as CXCL8 is a member of the CXC subfamily of chemokines and a major mediator of the inflammatory response. IL-8 functions as a chemotactic factor that attracts neutrophils as well as other granulocytes to sites of inflammation. It is secreted by several cell types including mononuclear macrophages, neutrophils, eosinophils, T-lymphocytes, fibroblast and epithelial cells in response to inflammatory stimuli. IL-8 also plays important functions in neutrophil activation, tumour migration, invasion and angiogenesis.

PODS are composed of a bioactive cargo protein within a polyhedrin crystal lattice, with polyhedrin as the main protein. The 25 µg SKU size indicates the amount of bioactive cargo, typically 0.5% to 5% of the total protein. For instance, a 25 µg SKU at a 2% cargo loading contains 1.25 mg of total protein. PODS enable sustained release of cargo proteins when proteases degrade the polyhedrin scaffold. Growth factors are not bioavailable until released, and their concentration in media depends on the amount added and the release rate. In 10% serum, peak levels are usually reached within 24-48 hours, with about 20% of the cargo becoming soluble.

PODS often require lower concentrations than conventional growth factors, as they maintain effective levels. Pre-incubating with serum or proteases enhances availability, while conventional factors can be added for immediate effects. Optimal amounts should be determined empirically.

PODS is a registered trademark of Cell Guidance Systems Ltd