Sigma-Aldrich Protein Delivery System Human Fgf-20 &

Protein delivery system, Human FGF-20, PODS polyhedrin protein co-crystalized with Human FGF-20. Fibroblast Growth Factor 20 (FGF-20) is a member of the fibroblast growth factor family, more specifically of the FGF-9 subfamily based on their structural and sequence similarities. It is expressed by a variety of cell types including fibroblasts, dopaminergic neurons, keratinocytes and epithelial cells. FGF-20, also regarded as a secreted neurotrophic factor, is involved in many biological processes, such as tumour growth, tissue repair, and embryonic development, specifically in the development of the central nervous system. It has shown to interact with FGF receptors 1c, 2c, 3b and 3c and 4c.

PODS are composed of a bioactive cargo protein within a polyhedrin crystal lattice, with polyhedrin as the main protein. The 25 µg SKU size indicates the amount of bioactive cargo, typically 0.5% to 5% of the total protein. For instance, a 25 µg SKU at a 2% cargo loading contains 1.25 mg of total protein. PODS enable sustained release of cargo proteins when proteases degrade the polyhedrin scaffold. Growth factors are not bioavailable until released, and their concentration in media depends on the amount added and the release rate. In 10% serum, peak levels are usually reached within 24-48 hours, with about 20% of the cargo becoming soluble.

PODS often require lower concentrations than conventional growth factors, as they maintain effective levels. Pre-incubating with serum or proteases enhances availability, while conventional factors can be added for immediate effects. Optimal amounts should be determined empirically.

PODS is a registered trademark of Cell Guidance Systems Ltd