Sigma-Aldrich Kimble(R) Kimax(R) Baffled Shake Flask

Oxygen intake is often the limiting factor for cell growth in the cultivation of microorganisms in Erlenmeyer flasks on a vibrating board or orbital shaker. The movement causes a liquid sickle to form when using Erlenmeyer flasks on a vibrator. The size of the sickle depends on the speed of the board and the vibration diameter. The greater the surface area of the contents, the greater the gas exchange area and therefore the potential oxygen intake. The speed and the associated oxygen intake can, however, only be increased to a limited extent. The baffled flask with baffles disrupts the laminar flow and produces a turbulent flow. The surface area of the liquid and the gas exchange area are increased, thereby increasing the oxygen intake. Laboratory trials have demonstrated that the oxygen intake is doubled by the baffles compared to a standard Erlenmeyer flask. The Erlenmeyer flasks with baffles from the DWK Life Sciences can be geometrically reproduced due to completely automated and mechanical production. The wall thickness of the flasks was increased to achieve an excellent mechanical stability and to guarantee a long service life of the products.

Cell culture, microbiology, fermentation

KIMBLE is a registered trademark of DWK Life Sciences

Kimax is a registered trademark of Gerresheimer Glass, Inc.