Sigma-Aldrich Anti-Yellow Fever Virus Clone 2D12.A

Yellow fever virus is an arbovirus of the Flavivirus genus. It is a single-stranded RNA virus, characterized by its spherical shape and an envelope derived from host cell membranes. The viral genome encodes a polyprotein (UniProt: P03314, Gene ID: 1502173) in Yellow fever virus (strain 17D), that is cleaved into structural proteins, including the capsid (C), surrounded by a lipid bilayer derived from the host cell, which contains two key envelope proteins: pre-membrane (prM), and envelope (E) proteins, essential for viral assembly and entry into host cells. The Envelope protein E plays a critical role in mediating fusion with host cell membranes, and its structure undergoes conformational changes upon exposure to neutral pH, facilitating viral entry. Clone 2D12.A is a mouse monoclonal antibody that specifically reacts with the envelope protein of the wild (Asibi) and vaccine strains of yellow fever virus. YFV is the cause of acute viral hemorrhagic disease transmitted by mosquitoes. YFV primarily infects hepatocytes, where it replicates and leads to cell death, contributing to the pathogenesis of yellow fever, a severe hemorrhagic disease fever, including fever, chills, loss of appetite, fatigue and in severe cases, liver failure, hemorrhage, and even death. (Ref.: Schlesinger, J., et al. (1983). Virulogy.125(1); 8-17.; Monath, T., et al.(1984). Am. J. Trop. Hyg. 33(1); 151-157.; Fernandez-Garcia, M., et al. (2016). mBio. 7(1); 1956-15.)

17D strain of Yellow fever virus

Quality Control Testing

Evaluated by ELISA with Yellow fever virus lysate.

Enzyme Immunoassay: A representative lot of this antibody detected Yellow fever virus Lysate from 17D strain of Yellow fever virus in ELISA application.

Tested Applications

Inhibition: A representative lot detected Yellow fever virus in Inhibition application (Schlesinger et al. (1983). Virology 125(1) 8-17).

Enzyme Immunoassay: A representative lot detected Yellow fever virus in ELISA application (Monath T. et al. (1984) Am J Trop Med Hyg 33(1) 151-7).

Immunocytochemistry Analysis: A representative lot detected Yellow fever virus in Immunocytochemistry application (Fernandez Garcia, MD., et al. (2016). mBio 7(1):e01956-15).

Flow Cytometry Analysis: A representative lot detected Yellow fever virus in Flow Cytometry application (Fernandez Garcia, MD., et al. (2016). mBio 7(1):e01956-15).

These cited applications are for the ascites version of this clone 2D12.A.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 2D12.A is a mouse monoclonal antibody that specifically reacts with the envelope protein of the wild (Asibi) and vaccine strains of Yellow fever virus. No cross-reactivity with other flaviviruses has been demonstrated.

Purified mouse monoclonal antibody IgG2a in PBS without azide.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.