Sigma-Aldrich Anti-Pgbd5 Clone 10A8-11-7-P-5

PiggyBac transposable element-derived protein 5 (UniProt: Q8N414; PiggyBac domain-related protein 5, PiggyBac transposase 5) is encoded by the PGBD5 gene (Gene ID: 79605) in human. PGBD5 is an endogenous human DNA transposase-derived protein that acts as a site-specific oncogenic mutator in human tumors, and able to promote DNA rearrangements of piggyBac-like transposons in human cells. PGBD5 is involved in genomic rearrangements affecting genomic stability but is also hypothesized to contribute to genomic diversity as well. Expression of PGBD5 is notably high in neuronal cells in the brain, in embryonic stem cells and certain cancer cells, suggesting a role in neural development and tumorigenesis. Its deficiency may cause disorder of intellectual ability, movement, and seizures. PGBD5 has been implicated in the development of pediatric and young-onset solid tumors, such as rhabdoid tumors and medulloblastomas, where it promotes oncogenic genomic DNA rearrangements. As a result, PGBD5 is being studied as a potential biomarker for certain cancers and as a target for therapeutic intervention. (Ref.: Henssen, A.G., et al. (2015). eLife 4:e10565; Henssen, A.G., et al. (2017). Nat Genet. 49:1005-1014).

KLH-conjugated linear peptide corresponding to 24 amino acids from the N-terminal of human PGBD5.

Quality Control Testing

Evaluated by Western Blotting in G-401 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PGBD5 in G-401 cell lysate.

Tested Applications

ChIP-seq: A representative lot detected PGBD5 in ChIP-seq application (Henssen, A.G., et al. (2020). Nat Genet. 52(11):1265).

Western Blotting Analysis: A representative lot detected PGBD5 in Western Blotting application (Henssen, A.G., et al. (2020). Nat Genet. 52(11):1265).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 10A8-11-7-P-5 is a mouse recombinant monoclonal antibody that detects PGBD5. It targets an epitope within 24 amino acids from the N-terminal region.

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.