Sigma-Aldrich Anti-Pilr# Clone 5F10 25 Mig

Paired immunoglobulin-like type 2 receptor alpha (UniProt: Q2YFS3; also known as Cell surface receptor FDF03, Inhibitory receptor PILR-alpha) is encoded by the Pilra gene (Gene ID: 231805) in murine species. These paired immunoglobulin-like receptor consist of highly related activating and inhibitory receptors and are widely involved in the regulation of the immune system. PILR-associating neural protein (PIANP) is a ligand for PILRA that facilitates the immune system regulation. It plays a pivotal role in regulating cell signaling via SHP-1 by balancing the PILRalpha-mediated inhibition and PILRbeta-mediated activation. PILR alpha is predominantly expressed in immune cells, including monocytes, macrophages, and dendritic cells. It is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-31), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 32-198), a transmembrane domain (aa 199-219), and a cytoplasmic domain (aa 220-302). The protein contains 2 cytoplasmic motif′s that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM motif 1&2), which are involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. Additionally, PILRalpha facilitates as a coreceptor that acquaints with glycoprotein B (gB) and assists in herpes simplex virus-1 entry. Further, it regulates the membrane fusion of the viral envelope with cellular membranes during virus entry and virus-induced cell-to-cell fusion. This Armenian hamster 5F10 antibody was developed and used in the study to evaluate antigen-independent functions of CD8. The study proves that CD8 is critical for the maintenance of CD8+ T cells in a physiologically quiescent state in peripheral lymphoid organs. PILR was identified as a ligand for CD8 in both mice and humans, and disruption of this interaction was able to break CD8+ T cell quiescence. This 5F10 antibody has been validated by Flow Cytometry and ELISA application. (Ref.:Zheng, L., et al. (2022). Science. 376(6596):996-1001).

Recombinant fragment corresponding to 170 amino acids from an extracellular domain of mouse PILRa.

Quality Control Testing

Evaluated by Flow Cytometry Analysis with J774.2 cells.

Flow Cytometry Analysis: 1.0 µg a representative lot detected PILR in one million J774.2 cells.

Tested Applications

ELISA: A representative lot detected PILR in ELISA application (Zheng, L., et al. (2022). Science. 376(6596):996-1001).

Flow Cytometry Analysis: A representative lot detected PILR in Flow Cytometry application (Zheng, L., et al. (2022). Science. 376(6596):996-1001).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 5F10 is an Armenian hamster monoclonal antibody that specifically detects PILR. It targets an epitope within 170 amino acids from the extracellular domain.

Purified armenian hamster monoclonal antibody IgG1 in PBS without preservatives.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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