Sigma-Aldrich Anti-Cd8# Clone 3D9 100 Mig

T-cell surface glycoprotein CD8 alpha chain (UniProt: P01731; also known as CD8a, T-cell surface glycoprotein Lyt-2) is encoded by the Cd8a (also known as Lyt-2, Lyt2) gene (Gene ID: ) in murine species. CD8 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-27), which is subsequently cleaved to form the mature protein that has an extracellular domain (aa 28-196), a helical domain (aa 197-217), and a cytoplasmic tail (aa 218-247). CD8 is generally present as a heterodimer of alpha and beta chains linked by two disulfide bonds, however, it can also form homodimers. The CD8 alpha/alpha homodimer is expressed on a subset of gamma/delta TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 plays a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. CD8 acts as a co-receptor for the T cell receptor (TCR) and like the TCR, it binds to class I MHC molecules displayed by antigen presenting cells (APC). CD8 is primarily expressed on the surface of cytotoxic T cells, but can also be found on thymocytes, natural killer cells, and some dendritic cell subsets. Altered CD8 expression or function are associated with various diseases, including autoimmune disorders and cancers. Dysregulation of CD8+ T-cell activity can lead to impaired immune responses or autoimmunity. (Ref.: Wang, R., et al., (2009), J Immunol, 183(4), 2533-2540; Sun, J., Kavathas, P.B., (1997), J Immunol, 158(9), 4199-4207; Classon, B.J., et al., (1992), Eur J Immunol, 22(5), 1527-1535)

Recombinant CD8 fragment corresponding to 150 amino acids from the extracellular domain of mouse CD8 .

Quality Control Testing

Flow Cytometry Analysis: 1.0 µg from a representative lot detected CD8 in one million C57BL/6 mouse splenocytes.

Tested Applications

Inhibits Activity/Function: A representative lot blocked CD8 PILR interaction in an Inhibition Assay (Zheng, L., et al. (2022). Science. 376(6596):996-1001).

Flow Cytometry Analysis: A representative lot detected CD8 in Flow Cytometry application (Zheng, L., et al. (2022). Science. 376(6596):996-1001).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 3D9 is a Armenian hamster monoclonal antibody that detects mouse CD8 . It targets an epitope within 150 amino acids from the extracellular domain.

Purified Armenian hamster monoclonal antibody IgG1 in PBS without preservatives.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.