Sigma-Aldrich Anti-Dnam-1/Cd226 Clone 11A8

CD226 antigen (UniProt: Q15762; also known as DNAX accessory molecule 1 (DNAM-1)) is encoded by the CD226 (also known as DNAM1) gene (Gene ID: 10666) in human. DNAM-1/CD226, is an immunoglobulin superfamily member that functions as a co-stimulatory receptor on various immune cells, including natural killer (NK) cells, T cells, and some myeloid cells. DNAM-1/CD226 is characterized by its extracellular Ig-like C2-type domains (aa 19-126 and aa 135-239), which facilitates its interaction with ligands such as CD112 and CD155. This interaction is crucial for mediating NK cell and cytotoxic T lymphocyte (CTL) activities against tumor cells and virus-infected cells. DNAM-1/CD226 is predominantly expressed on NK cells, CD8+ T cells, and subsets of CD4+ T cells. DNAM-1/CD226 plays a role as a co-stimulatory molecule is pivotal in immune surveillance, particularly in the context of tumor immunology. DNAM-1′s interaction with its ligands facilitates the activation and proliferation of NK and T cells, thus promoting the clearance of malignant cells. DNAM-1/CD226 has been implicated in various cancers and autoimmune disorders. Its expression and function are often altered in malignancies, affecting the immune system′s ability to recognize and destroy tumor cells. Loss of DNAM-1/CD226 contributes to CD8+ T cell exhaustion in chronic HIV-1 infection. (Ref.: Vo, A.V., et al., (2016), Immunology Letters, 169, 72-80; Martinet, L., et al., (2015), Cancer Immunology Research, 3(4), 328-339; Tahara-Hanaoka, S., et al., (2004), International Immunology, 16(4), 533-538; Cella, M., et al. (2010). Eur J Immunol. 40(4):949-54)

Human NK Cells.

Quality Control Testing

Evaluated by Flow Cytometry in human peripheral blood mononuclear cells (PBMC).

Flow Cytometry Analysis: 1.0 µg from a representative lot detected DNAM-1/CD226 in one million human peripheral blood mononuclear cells (PBMC).

Tested Applications

Flow Cytometry Analysis: A representative lot detected DNAM-1/CD226 in Flow Cytometry application (Fuchs, A., et al. (2004). J Immunol. 172(7), 3994-8; Cella, M., et al. (2010). Eur J Immunol. 40(4), 949-54; Guo, H., et al. (2015). Mol Cell Biol. 35(1):41-51).

Activity Assay: A representative lot blocked DNAM-1/CD226 activity in redirected cytotoxicity assay (Cella, M., et al. (2010). Eur J Immunol. 40(4):949-54).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 11A8 is a mouse monoclonal antibody that detects DNAM-1/CD226. It targets an epitope from the extracellular domain.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.