Sigma-Aldrich Anti-Aminopeptidase A/Enpep Clone 6C3

Glutamyl aminopeptidase (UniProt: P16406; also known as EC:3.4.11.7, CD249, Aminopeptidase A (AP-A), BP-1/6C3 antigen, EAP, gp160) is encoded by the Enpep gene (Gene ID: 13809) in murine species. Aminopeptidase A/ENPEP is a type II integral membrane endopeptidase characterized by a large extracellular C-terminal domain that contains a zinc-binding motif typical of zinc-dependent metallohydrolases. Aminopeptidase A/ENPEP is involved in the regulation of blood pressure and blood vessel formation by cleaving N-terminal glutamate residues from peptides such as angiotensin II, thereby modulating the renin-angiotensin system. It also plays a role in the final digestion of peptides generated from the hydrolysis of proteins by gastric and pancreatic proteases, and is involved in processing various peptide hormones, neuropeptides, and chemokines. Aminopeptidase A/ENPEP is expressed at high levels in the epithelial cells of the kidney glomerulus and proximal tubule cells. Its dysregulation has been implicated in hypertension and cardiovascular diseases due to its role in the renin-angiotensin system. Recent studies have also suggested its potential involvement in cancer progression and immune modulation, highlighting its expression as a predictor for immune checkpoint inhibitor therapy. (Ref: Shen, C., et al., (2015), Biochem Biophys Res Commun, 460(2), 261-267)

Cytotoxic T cells and L1-2 stimulator cells.

Quality Control Testing

Evaluated by Flow Cytometry in L1.2 cells.

Flow Cytometry Analysis: A 1:1,000 dilution of this antibody detected Aminopeptidase A/ENPEP in one million L1.2 cells.

Tested Applications

Immunocytochemistry Analysis: A representative lot detected Aminopeptidase A/ENPEP in Immunocytochemistry application (Pillemer, E., et al. (1984). Proc Natl Acad Sci USA. 81(14):4434-8).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Aminopeptidase A/ENPEP in Immunoprecipitation application (Pillemer, E., et al. (1984). Proc Natl Acad Sci USA. 81(14):4434-8).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 6C3 is a rat monoclonal antibody that detects mouse Aminopeptidase A/ENPEP. It targets an epitope within the extracellular domain.

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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