Sigma-Aldrich Anti-Dectin-2 Clone 17A1D10 25 Mig

C-type lectin domain family 6 member A (UniProt: Q6EIG7; also known as C-type lectin superfamily member 10, Dendritic cell-associated C-type lectin 2, DC-associated C-type lectin 2, Dectin-2) is encoded by the Clec6a (also known as CLECSF10, DECTIN2) gene (Gene ID: ) in human. Dectin-2 plays a critical role in the innate and adaptive immune system by recognizing pathogen-associated molecular patterns (PAMPs) and initiating immune responses. This receptor is an ITAM-coupled C-type lectin receptor (CLR); a single-pass type II transmembrane protein. It features a carbohydrate recognition domain (CRD) that allows it to bind specifically to high-mannose polysaccharides found on the surface of various pathogens, including fungi and some bacteria. It is characterized by a single extracellular (aa 42-209) C-type lectin-like domain (CTLD) connected by a transmembrane region (aa 22-41) to a short cytoplasmic tail (aa 1-20) that lacks a clear intracellular signaling motif. Association of Dectin-2 with adaptor proteins, such as the Fc receptor (FcR ) chain or DNAX-activation protein 12 (DAP12), is required for the engagement to Syk pathway, leading to the activation of NF- B and the production of pro-inflammatory cytokines. Its expression is particularly high in the spleen and lymph nodes and primarily expressed on dendritic cells and macrophages. Dectin-2 has been implicated in various diseases, particularly those involving fungal infections due to its role in recognizing fungal cell wall components. dysregulation of Dectin-2 signaling has been associated with inflammatory and autoimmune diseases, highlighting its importance in maintaining immune homeostasis. Dectin-2 plays critical roles in the pathogenesis of allergic diseases. (Ref.: Malamud, M., and Brown, G. D., (2024) EMBO Rep, 25, 5239-5264); Chen, M.H., et al., (2019) J. Biomed. Sci. 26:97; Angelina, A., et al., (2023) Curr Allergy and Asthma Rep. 23, 141-151)

hFc-tagged, full-length human Dectin-2.

Quality Control Testing

Evaluated by Enzyme Immunoassay (ELISA) with hFc-tagged, full-length, recombinant, human Dectin-2.

Enzyme Immunoassay (ELISA): A representative lot of this antibody detected hFc-tagged, full-length, recombinant, human Dectin-2.

Tested Applications

Surface plasmon resonance: Chen, M-H., et al. (2019). J Biomed Sci. 26(1):97.

ELISA Analysis: A representative lot detected Dectin-2 in ELISA application (Chen, M-H., et al. (2019). J Biomed Sci. 26(1):97).

Inhibition Assay: A representative lot of this antibody inhibited house dust mite binding to Dectin-2. (Chen, M-H., et al. (2019). J Biomed Sci. 26(1):97).

Flow Cytometry Analysis: A representative lot detected Dectin-2 in Flow Cytometry application (Chen, M-H., et al. (2019). J Biomed Sci. 26(1):97).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 17A1D10 is a mouse monoclonal antibody that detects Dectin-2.

Purified mouse monoclonal antibody IgG2a in PBS without preservatives.

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.